Directed Evolution of an EnantioselectiveBacillus subtilisLipase
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چکیده
منابع مشابه
Directed evolution of an RNA enzyme.
An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10(13) variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl2 concentration,...
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ژورنال
عنوان ژورنال: Biocatalysis and Biotransformation
سال: 2003
ISSN: 1024-2422,1029-2446
DOI: 10.1080/1024242031000110847